FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_68.351000000602f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_68.351000000602f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62777
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT5240.834700606910174No Hit
AGTCTCCTACGTCTGTCTCTTATACAC4560.7263806808225943No Hit
AGTCTCCTACGCTGTCTCTTATACACA1310.2086751517275435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTT558.480129E-921.0215518
ATCTTTT558.480129E-921.0215518
AAATTTT507.2006515E-821.0215518
TGCGTTT507.2006515E-821.0215517
GCGATTT303.8499254E-421.0215517
AATTTTT2400.021.0215519
ATGATTT303.8499254E-421.0215517
CAACTTT250.003324888921.0215517
TATGTTT250.003324888921.0215517
TGATTTT601.0004442E-921.0215518
CAATTTT601.0004442E-921.0215518
GGTTCTG250.003324888921.0215519
GGTTTTT7600.021.0215519
CGGATTT303.8499254E-421.0215517
TAGTTTT1000.021.0215518
GCAATTT303.8499254E-421.0215517
GACATTT250.003324888921.0215517
CGGGTTT950.021.0215517
GCAGTTT507.2006515E-821.0215517
TATCTTT303.8499254E-421.0215517
Produced by FastQC (version 0.11.2)