FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_67.351000000602de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_67.351000000602de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54949
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTGGGGGCTGTCTCTTA560.10191268266938433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCGC1350.021.018231
TCAGAGC47950.021.018231
TCCGAGC2700.021.018231
GTGTTTT1500.020.9990918
CATTGTG1500.020.999099
CATTGTC800.020.999099
GGCATTT250.003340343520.9990917
AGCTTTT507.241033E-820.9990918
CATTGGG3000.020.999099
CATTGCG1150.020.999099
CATTGCC800.020.999099
CATTGAT1150.020.999099
CATTGAG1550.020.999099
AAATTTT303.869363E-420.9990918
TGCGTTT250.003340343520.9990917
ATTAGTA303.869363E-420.9990910
ATGATTT250.003340343520.9990917
ATTAGGG751.8189894E-1220.9990910
ATTAGGC405.2593787E-620.9990910
ATTAGGA250.003340343520.9990910
Produced by FastQC (version 0.11.2)