FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_66.351000000602b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_66.351000000602b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37378
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA2220.593932259617957No Hit
GCATTGGATACCGGCTGTCTCTTATAC1880.5029696612980898No Hit
GCATTGGATACCCTGTCTCTTATACAC1480.3959548397453047No Hit
GCATTGGATACCTGCTGTCTCTTATAC880.23543260741612712No Hit
GCATTGGATACCGCCTGTCTCTTATAC820.21938038418320938No Hit
GCATTGGATACCAGCTGTCTCTTATAC800.21402964310557016No Hit
GCATTGGATACCCGCTGTCTCTTATAC780.20867890202793085No Hit
GCATTGGATACCGTCTGTCTCTTATAC740.19797741987265235No Hit
GCATTGGATACCACCTGTCTCTTATAC600.1605222323291776No Hit
GCATTGGATACCATCTGTCTCTTATAC560.1498207501738991No Hit
GCATTGGATACCCCCTGTCTCTTATAC530.1417946385574402No Hit
GCATTGGATACCCTGTCTCTTCTACAC530.1417946385574402No Hit
GCATTGGATACCCCTGTCTCTTATACA500.13376852694098132No Hit
GCATTGGATACCGACTGTCTCTTATAC430.11504093316924394No Hit
GCATTGGATACCGCTGTCTCTTATACA420.11236556263042431No Hit
GCATTGGATACTGTCTCTTATACACAT400.10701482155278508No Hit
GCATTGGATACCTCCTGTCTCTTATAC400.10701482155278508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTGG32900.021.0268121
GCTTTGG950.021.0268121
GTATTGG800.021.026811
TTGTTTT507.0871465E-820.99866318
ACCGGTG507.0871465E-820.99866310
ACCGGGT456.0557613E-720.99866310
ACCGGGC507.0871465E-820.99866310
ACCGGGA456.0557613E-720.99866310
ACCGGCT751.8189894E-1220.99866310
ACCGGCA456.0557613E-720.99866310
ACCGGAC250.003320976920.99866310
GCCTTTT250.003320976920.99866318
ACGTTTT456.0557613E-720.99866318
GTCGTTT250.003320976920.99866317
GATACCG14400.020.9986637
GATGTTT250.003320976920.99866317
GCGGTTT456.0557613E-720.99866317
TGTTCTG250.003320976920.99866319
GTATTTT507.0871465E-820.99866318
CAATTTT456.0557613E-720.99866318
Produced by FastQC (version 0.11.2)