FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_64.3510000006028e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_64.3510000006028e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51134
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC4410.8624398638870419No Hit
GAACACCATAGCGGCTGTCTCTTATAC3090.6042945985058865No Hit
GAACACCATAGCGCCTGTCTCTTATAC1870.3657057926233035No Hit
GAACACCATAGCCTGTCTCTTCTACAC1830.35788320882387453No Hit
GAACACCATAGCAGCTGTCTCTTATAC1530.2992138303281574No Hit
GAACACCATAGCGTCTGTCTCTTATAC1320.2581452653811554No Hit
GAACACCATAGCGACTGTCTCTTATAC1120.21903234638401062No Hit
GAACACCATAGCTGCTGTCTCTTATAC1110.21707670043415342No Hit
GAACACCATAGCACCTGTCTCTTATAC1070.20925411663472443No Hit
GAACACCATAGCATCTGTCTCTTATAC1060.2072984706848672No Hit
GAACACCATAGCTGTCTCTTATACACA1050.20534282473500995No Hit
GAACACCATAGCCCCTGTCTCTTATAC830.16231861383815074No Hit
GAACACCATAGCCGCTGTCTCTTATAC820.1603629678882935No Hit
GAACACCATAGCCCTGTCTCTTATACA640.12516134079086322No Hit
GAACACCATAGCTCCTGTCTCTTATAC600.11733875699143426No Hit
GAACACCATAGCAACTGTCTCTTATAC550.10756052724214808No Hit
GAACACCATAGCTACTGTCTCTTATAC530.1036492353424336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1300.021.00000218
TAGCGGC1500.021.0000029
TAGCGCG1500.021.0000029
TAGCGCC651.1823431E-1021.0000029
GCCTTTT651.1823431E-1021.00000218
CAGTTTT751.8189894E-1221.00000218
TAGCCTT558.487405E-921.0000029
TAGCTTA651.1823431E-1021.0000029
TAGCCGC751.8189894E-1221.0000029
TAGCTCT651.1823431E-1021.0000029
TAGCACG558.487405E-921.0000029
TAGCACA751.8189894E-1221.0000029
TAGCAAC651.1823431E-1021.0000029
CGGGTTT558.487405E-921.00000217
ATAGCTT3000.021.0000028
ATAGCTG4400.021.0000028
AGCGGTG651.1823431E-1021.00000210
ATAGCCC1500.021.0000028
ATAGCAC2600.021.0000028
AGCGGGG1300.021.00000210
Produced by FastQC (version 0.11.2)