FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_63.35100000060264.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_63.35100000060264.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53370
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA3250.6089563425145212No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT1300.24358253700580854No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC900.16863406408094433No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT850.15926550496533634No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT770.14427581038036352No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT680.12741240397226905No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA610.11429642121041785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTCT303.82666E-421.03192520
ATTTCTG558.360075E-921.03192520
ATCTGTC1100.021.03192520
ATACACA354.445363E-521.03192521
CTTCTGT354.445363E-521.03192520
TTTTCTG651.1641532E-1021.03192321
TGGCATA47650.021.0121971
TGGTTTT950.021.01219618
TTGCATA800.021.0121961
GCCTTTT751.8189894E-1221.01219618
CAGTTTT751.8189894E-1221.01219618
GTCATTT303.8510552E-421.01219617
AAATTTT405.226293E-621.01219618
ACGTTTT800.021.01219618
GTCGTTT303.8510552E-421.01219617
GGCGTTT950.021.01219617
GGTCTTT405.226293E-621.01219617
TTATTTT303.8510552E-421.01219618
GATGTTT303.8510552E-421.01219617
TGTTTTT3300.021.01219619
Produced by FastQC (version 0.11.2)