FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_62.3510000006024b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_62.3510000006024b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47712
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA3280.687458081824279No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT1170.24522132796780685No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC1050.22007042253521128No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT620.12994634473507713No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC530.11108316566063045No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC520.10898725687458083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTG800.021.0000025
GCGGGGA507.187555E-821.00000211
GCGGGCC405.2334744E-621.00000211
GCGGGAT250.003333148321.00000211
TTGCATG558.451025E-921.0000029
ACGCTTT250.003333148321.00000217
CAGTTTT651.1823431E-1021.00000218
TTGCAGT800.021.0000029
TTGCAGG1100.021.0000029
TTGCAGC507.187555E-821.0000029
TTGCAGA651.1823431E-1021.0000029
TTGCACT250.003333148321.0000029
TGCGCTG250.003333148321.00000210
TGCGCTC250.003333148321.00000210
TGCGCGG405.2334744E-621.00000210
TGCGCGC558.451025E-921.00000210
TGCGCGA507.187555E-821.00000210
TTTCTTT250.003333148321.00000217
TGCGCCT507.187555E-821.00000210
TGCGCCC250.003333148321.00000210
Produced by FastQC (version 0.11.2)