FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_6.3510000005fb44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_6.3510000005fb44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA391.5192832099727307No Hit
CTGATCGTAGCACCTGTCTCTTATACA200.7791195948578107No Hit
CTGATCGTAGCAGCTGTCTCTTATACA90.3506038176860148No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT70.2726918582002337No Hit
CTGATCGTAGCCTGTCTCTTATACCCA60.23373587845734323No Hit
CTGATCGTAGCTGTCTCTTATACACAT60.23373587845734323No Hit
CTGATCGTAGCACTGTCTCTTATACAC50.19477989871445267No Hit
CTGATCGTAGCACCTGTCTCTTATACC40.15582391897156214No Hit
CTGATCGTAGCCTGTCTCTTATACCCC40.15582391897156214No Hit
CTGATCGTAGCAGGCCGGGATTTTTTT40.15582391897156214No Hit
CTGATCGTAGCAGGGCGGGGTTTTTTT40.15582391897156214No Hit
CTGATCGTAGCCTGTCTCTTATACACC40.15582391897156214No Hit
CTGATCGTAGCAGGGTGGGGTTTTTTT30.11686793922867161No Hit
CTGATCGTAGCAGTGGGGGATTTTTTT30.11686793922867161No Hit
CTGATCGTAGCATCTGTCTCTTATACA30.11686793922867161No Hit
CTGATCGTAGCAGGCGGGGGTTTTTTT30.11686793922867161No Hit
CTGATCGTAGCCTGTCTCTTCTACACA30.11686793922867161No Hit
CTGATCGTAGCATGTGCTGTCTCTTAT30.11686793922867161No Hit
CTGATCGTAGCAGGCGGGGGTCTGTCT30.11686793922867161No Hit
CTGATCGTAGCAACACCTGTCTCTTAT30.11686793922867161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT1650.021.021
GATCGTA2400.021.03
GTTTTTT750.021.020
GGTTTTT402.719351E-621.019
CGTAGCA2550.021.06
TAGCAGT302.6474395E-421.08
TAGCAGG402.719351E-621.08
TAGCAGA302.6474395E-421.08
TAGCACG402.719351E-621.08
TCGTAGC2550.021.05
ATCGTAG2550.021.04
GTAGCAT352.7053582E-521.07
GTAGCAG1200.021.07
GTAGCAC602.3101165E-1021.07
GTAGCAA402.719351E-621.07
CTTTTTT402.719351E-621.020
GGGTTTT250.002551460920.99999818
TGATCGT2250.020.9999982
CTGATCG2250.020.9999981
TAGCAAG250.002551460920.9999988
Produced by FastQC (version 0.11.2)