FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_59.351000000601df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_59.351000000601df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66596
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA860.1291368850982041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT354.5195407E-521.00000218
GCCTTTT601.0168151E-921.00000218
CAGTCTA701.4551915E-1121.0000029
GTGTTGA303.8801914E-421.00000211
CAGTTGC1100.021.0000029
CAGTCCT701.4551915E-1121.0000029
CAGTCCG850.021.0000029
CAGTCCC701.4551915E-1121.0000029
CAGTTCG1100.021.0000029
CAGTCAT558.60382E-921.0000029
TTCGTTT303.8801914E-421.00000217
GCGATTT303.8801914E-421.00000217
AGTAGTA303.8801914E-421.00000210
AGGATTT303.8801914E-421.00000217
AGTAGGC303.8801914E-421.00000210
AGTAGAG354.5195407E-521.00000210
TCGGTTT354.5195407E-521.00000217
CAGTAGT850.021.0000029
CAGTACC354.5195407E-521.0000029
CAGTAAT701.4551915E-1121.0000029
Produced by FastQC (version 0.11.2)