FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_57.3510000006019c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_57.3510000006019c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43105
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTACGTAGGCTGTCTCTTATACA4210.9766848393457835No Hit
TACGGTACGTAGCCTGTCTCTTATACA1950.4523837141862893No Hit
TACGGTACGTAGTCTGTCTCTTATACA1690.3920658856281174No Hit
TACGGTACGTAGACTGTCTCTTATACA1600.3711866372810579No Hit
TACGGTACGTAGCTGTCTCTTATACAC1360.3155086416888992No Hit
TACGGTACGTAGGGCTGTCTCTTATAC920.2134323164366083No Hit
TACGGTACGTAGAGCTGTCTCTTATAC630.14615473842941654No Hit
TACGGTACGTAGCTGTCTCTTCTACAC600.1391949889803967No Hit
TACGGTACGTAGGGGCTGTCTCTTATA580.1345551560143835No Hit
TACGGTACGTAGACCTGTCTCTTATAC560.12991532304837025No Hit
TACGGTACGTAGGCCTGTCTCTTATAC470.10903607470131074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT8900.021.01858520
TTGTTTT558.307325E-921.01858318
GGGTTTT1650.021.01858318
CAGTTTT800.021.01858318
TATATTT354.4415567E-521.01858317
AAATTTT651.1459633E-1021.01858318
AATTTTT2300.021.01858319
ACGTTTT250.00331089821.01858318
TTTCTTT250.00331089821.01858317
TGTCTGT558.307325E-921.01858319
TCGGTTT250.00331089821.01858317
TTATTTT558.307325E-921.01858318
TGATTTT507.0798706E-821.01858318
CGCTTTT250.00331089821.01858318
TGTTTTT3000.021.01858319
GGATTTT1000.021.01858318
GGTTCTG250.00331089821.01858319
CATTTTT1450.021.01858319
CCGTTTT354.4415567E-521.01858318
TAGTTTT701.2732926E-1121.01858318
Produced by FastQC (version 0.11.2)