FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_55.35100000060165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_55.35100000060165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66728
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC2330.3491787555448987No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT760.11389521640091116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC1050.021.0150051
CATCAGC900.021.0150051
CCTCAGC1100.021.0150051
CTTAAGC354.494864E-521.0150051
CTTCCGC3600.021.0150051
CTTCAGC52750.021.0150031
CTCCAGC751.8189894E-1221.0150031
CTTCTGC303.8616074E-421.0150031
ACAGCAA1050.020.9992523
GTGTTTT1650.020.99925218
CAATGCG26400.020.9992527
TACAGCA1050.020.9992522
CATTTTT2900.020.99925219
ATGCGCG1900.020.9992529
ATGCGCC1650.020.9992529
ATGCGAG2100.020.9992529
ACTTTTT1900.020.99925219
ATGCCTG1450.020.9992529
ATGCCGC1050.020.9992529
ATGCTGC1250.020.9992529
Produced by FastQC (version 0.11.2)