FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_54.3510000006014c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_54.3510000006014c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49488
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA4320.8729388942774006No Hit
CTGATCGTAGCACCTGTCTCTTATACA1520.30714516650501134No Hit
CTGATCGTAGCAGCTGTCTCTTATACA1050.212172647914646No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT600.12124151309408343No Hit
CTGATCGTAGCAGGGGCTGTCTCTTAT580.11720012932428063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT2650.021.018222
CGGATCG2650.021.018221
TGATCGT42550.021.0182192
GATCGTA46600.021.0182193
CTGATCG42650.021.0182191
GCTCGTA1450.021.0182193
TGCTCGT1450.021.0182192
CTGCTCG1450.021.0182191
AGATCGT558.385541E-921.0182192
CCGATCG850.021.0182191
CGATCGT850.021.0182192
TATCGTA751.8189894E-1221.0182173
CTTATCG751.8189894E-1221.0182171
GCAGGGG2250.020.9969710
ACTGTTT354.4943423E-520.99696717
AGGGGGC354.4943423E-520.99696712
GCCTTTT950.020.99696718
AGCTTTT558.483767E-920.99696718
GAAATTT354.4943423E-520.99696717
TGCGTTT405.246484E-620.99696717
Produced by FastQC (version 0.11.2)