FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_50.351000000600b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_50.351000000600b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42973
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA830.1931445326135015No Hit
GGTAGTGAGCTAGGGGCTGTCTCTTAT480.11169804295720567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT609.877112E-1021.00000218
TAGCGGG303.8492773E-421.00000211
GTCATTT303.8492773E-421.00000217
TAGGAGG456.0961065E-721.00000211
CCAGTTT303.8492773E-421.00000217
GCTAGGG3600.021.0000029
GCTAGAG1200.021.0000029
TTTCTGT456.0961065E-721.00000221
AGCTATA1200.021.0000028
TCGGTTT303.8492773E-421.00000217
CGCTTTT609.877112E-1021.00000218
GGATTTT900.021.00000218
GTTTCTG303.8492773E-421.00000220
CTAAGGG609.877112E-1021.00000210
CTAGGGG1800.021.00000210
CTAGGGA609.877112E-1021.00000210
GCTATGG1800.021.0000029
CTAGGCG609.877112E-1021.00000210
CTAGGCC303.8492773E-421.00000210
GCTACAG456.0961065E-721.0000029
Produced by FastQC (version 0.11.2)