FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_5.3510000005fb2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_5.3510000005fb2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC161.21580547112462No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC60.4559270516717325No Hit
GAGCTACGTCTATCCTGTCTCTTATAC40.303951367781155No Hit
GAGCTACGTCTACGCTGTCTCTTATAC40.303951367781155No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT40.303951367781155No Hit
GAGCTACGTCTCTGTCTCTTCTACACA40.303951367781155No Hit
GAGCTACGTCTATGGCTGTCTCTTATA40.303951367781155No Hit
GAGCTACGTCTACCCCTGTCTCTTATA40.303951367781155No Hit
GAGCTACGTCTAGGGTCTGTCTCTTAT30.22796352583586624No Hit
GAGCTACGTCTAGGGGGCTGTCTCTTA30.22796352583586624No Hit
GAGCTACGTCTATAGCTGTCTCTTATA30.22796352583586624No Hit
GAGCTACGTCTAACCTGTCTCTTATAC30.22796352583586624No Hit
GAGCTACGTCTAGCCTGTCTCTTATAC30.22796352583586624No Hit
GAGCTACGTCTAAGGCTGTCTCTTATA30.22796352583586624No Hit
GAGCTACGTCTAGGGCGGGGTTTTTTT30.22796352583586624No Hit
GAGCTACGTCTAGTGGGTACTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTATGGGCTGTCTCTTAT20.1519756838905775No Hit
GAGCTACGTCTATTCTGTCTCTTATAC20.1519756838905775No Hit
GAGCTACGTCTACGGCTGTCTCTTATA20.1519756838905775No Hit
GAGCTACGTCTAAGCCTGTCTCTTATA20.1519756838905775No Hit
GAGCTACGTCTACACCTGTCTCTTATA20.1519756838905775No Hit
GAGCTACGTCTAAGCTGTCTCTTATAC20.1519756838905775No Hit
GAGCTACGTCTAGGCCTTGATTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAGAGCCCCGTCTGTCT20.1519756838905775No Hit
GAGCTACGTCTACGAGTTTATTTTTTT20.1519756838905775No Hit
GAGCTACGTCTGTCTCTTATCCACATC20.1519756838905775No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA20.1519756838905775No Hit
GAGCTACGTCTACCTGTCTCTTATACA20.1519756838905775No Hit
GAGCTACGTCTACGTTAGTCTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTACGGGCTGTCTCTTAT20.1519756838905775No Hit
GAGCTACGTCTATGCCTGTCTCTTATA20.1519756838905775No Hit
GAGCTACGTCTATGCCTGTCTCTTATC20.1519756838905775No Hit
GAGCTACGTCTAACCTGGGGCTGTCTC20.1519756838905775No Hit
GAGCTACGTCTGTCTCTTATACACCTC20.1519756838905775No Hit
GAGCTACGTCTAAGGCCTGTCTCTTAT20.1519756838905775No Hit
GAGCTACGTCTAATCAGGTGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAAGTCACAGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTACATTGTGATTTTTTT20.1519756838905775No Hit
GAGCTACGTCTATATAAAGTTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAGGGCCGCATTTTTTT20.1519756838905775No Hit
GAGCTACGTCTCTGTCTCTTATACACA20.1519756838905775No Hit
GAGCTACGTCTAAGGATCAATTTTTTT20.1519756838905775No Hit
GAGCTACGTCTACGGGGTTGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAAGTCCTGTCTCTTAT20.1519756838905775No Hit
GAGCTACGTCTATAGCAGGGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAATGGGCGCTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTACAGGCAAGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTATGGGGGGGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTACCTCGGCATTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAGCCCTGTCTCTTATA20.1519756838905775No Hit
GAGCTACGTCTAGGACGGGGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTACCATAATCTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAATTTGGTGTTTTTTT20.1519756838905775No Hit
GAGCTACGTCTAAGGTCGGGTTTTTTT20.1519756838905775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTA1250.021.06
AGCTACG1200.021.02
TTTTTTT700.021.021
GTTTTTT301.7362414E-421.020
GAGCTAC1200.021.01
GCTACGT1250.021.03
TACGTCT1300.021.05
CTACGTC1300.021.04
CGTCTAA301.7362414E-421.07
GTCTAAT250.001900673820.9999988
CGTCTAT451.01237674E-720.9999987
CGTCTAG250.001900673820.9999987
CGTCTAC250.001900673820.9999987
Produced by FastQC (version 0.11.2)