FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_48.35100000060073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_48.35100000060073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC521.9756838905775076No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC260.9878419452887538No Hit
TAAGCGTGGAACAGCTGTCTCTTATAC40.1519756838905775No Hit
TAAGCGTGGAACTGGGGGGGTTTTTTT30.11398176291793312No Hit
TAAGCGTGGAACTGTCTCTTATACACA30.11398176291793312No Hit
TAAGCGTGGAACTGCTGTCTCTTATAC30.11398176291793312No Hit
TAAGCGTGGAACAGGGGGGGTTTTTTT30.11398176291793312No Hit
TAAGCGTGGAACGCGGGGGGTTTTTTT30.11398176291793312No Hit
TAAGCGTGGAACGGGGGGGCTTTTTTT30.11398176291793312No Hit
TAAGCGTGGAACCTGTCTCTTCTACCC30.11398176291793312No Hit
TAAGCGTGGAACGGCGGGGGTTTTTTT30.11398176291793312No Hit
TAAGCGTGGAACGTGCGGGGTTTTTTT30.11398176291793312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT2350.021.01
GCGTGGA2600.021.04
CGTGGAA2600.021.05
AAGCGTG2350.021.02
TGGAACC302.6687197E-421.07
AGCGTGG2350.021.03
GGAACGG402.7580645E-621.08
GTGGAAC2500.021.06
GGTTTTT502.6613634E-820.99999819
TGGAACT552.5411282E-920.9999987
TGGAACG1100.020.9999987
TGGAACA552.5411282E-920.9999987
GGAACTG250.002565891720.9999988
GGAACGC250.002565891720.9999988
GGAACGA250.002565891720.9999988
TTTTTTT1600.019.687521
ATTTTTT507.570725E-718.920
GTTTTTT1000.017.84999820
GCTGTCT300.007389962717.520
Produced by FastQC (version 0.11.2)