FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_46.35100000060030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_46.35100000060030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12305
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA720.5851279967492888No Hit
TACTGCAAGGAGGCTGTCTCTTATACA360.2925639983746444No Hit
TACTGCAAGGAGGGGGGGCTGTCTCTT190.15440877691995125No Hit
TACTGCAAGGAGCCTGTCTCTTATACA160.13002844372206418No Hit
TACTGCAAGGAGCTGTCTCTTATACAC140.11377488825680616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGG303.6372754E-421.0000029
TGTTTTT659.094947E-1121.00000219
GGTTTTT1050.021.00000219
CATTTTT303.6372754E-421.00000219
GGAGGGC404.730231E-621.0000029
GGAGGGA303.6372754E-421.0000029
AAGGAGT2100.021.0000027
AAGGAGG5850.021.0000027
CGGTTTT303.6372754E-421.00000218
GCTTTTT404.730231E-621.00000219
GGAGTGT404.730231E-621.0000029
GGAGTAG303.6372754E-421.0000029
AGGAGGG2400.021.0000028
TGGTTTT250.00319584821.018
AGGGGGG250.00319584821.011
GGAGAGC250.00319584821.09
ACTGCAA11650.021.02
GCAAGGA12050.021.05
AATTTTT506.1567334E-821.019
TGCAAGG12050.021.04
Produced by FastQC (version 0.11.2)