FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_45.35100000060016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_45.35100000060016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6779
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTGTCTCTTATACACATC180.2655258887741555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG6250.021.0000023
TCTAGAC250.00304848921.0000022
GCTAGGT250.00304848921.0000029
ATCTAGA250.00304848921.0000021
GGATTTT250.00304848921.00000218
CGCTATG505.163929E-821.0000028
CGCTAGG750.021.0000028
CGCTAGC555.677066E-921.0000028
CGCTACG505.163929E-821.0000028
CGCTACC555.677066E-921.0000028
CGCTAAG505.163929E-821.0000028
GACGCTA6650.021.0000026
GCTAAGA250.00304848921.0000029
AGACGCT250.00304848921.0000025
GCTCGAC250.00304848921.0000022
CTAGACG250.00304848921.0000023
TAGACGC250.00304848921.0000024
ACGCTAT1650.021.07
ACGCTAG2050.021.07
ACGCTAC1550.021.07
Produced by FastQC (version 0.11.2)