FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_4.3510000005fb00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_4.3510000005fb00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC501.266464032421479No Hit
AGACGTCGATACCTGTCTCTTCTACAC280.7092198581560284No Hit
AGACGTCGATACACCTGTCTCTTATAC70.1773049645390071No Hit
AGACGTCGATACGGGGGGGGTTTTTTT60.1519756838905775No Hit
AGACGTCGATACGCTGTCTCTTATACA60.1519756838905775No Hit
AGACGTCGATACGGGGGTGGTTTTTTT50.12664640324214793No Hit
AGACGTCGATACGGCGGGGGTTTTTTT50.12664640324214793No Hit
AGACGTCGATACGGGGGGGCTTTTTTT40.10131712259371835No Hit
AGACGTCGATACGGGGGGCTGTCTCTT40.10131712259371835No Hit
AGACGTCGATACGGCTGTCTCTTATAC40.10131712259371835No Hit
AGACGTCGATACGGGGCGGGTTTTTTT40.10131712259371835No Hit
AGACGTCGATACGGGCAGGGTTTTTTT40.10131712259371835No Hit
AGACGTCGATACTGGGTCTGTCTCTTA40.10131712259371835No Hit
AGCCGTCGATACCTGTCTCTTCTACAC40.10131712259371835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT250.002820193521.018
CGATACT604.0563464E-1021.07
CGATACG1900.021.07
CGATACC850.021.07
TCGATAC3900.021.06
GATACGT503.8440703E-821.08
GATACGG850.021.08
GATACGC503.8440703E-821.08
GATACCG303.050626E-421.08
GATACCC250.002820193521.08
GATACAG403.4876957E-621.08
ACGTCGA3350.021.03
GTCGTCG250.002820193521.02
TTTTTTT2500.021.021
TGTTTTT353.275441E-521.019
GTCGATA3900.021.05
GTTTTTT1000.021.020
GGTTTTT303.050626E-421.019
TGCTTTT303.050626E-421.018
AGACGTC3350.021.01
Produced by FastQC (version 0.11.2)