FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_39.3510000005ff69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_39.3510000005ff69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA110.4286827747466875No Hit
ACTTCGTCAGCTGTCTCTTATACACAT100.3897116134060795No Hit
ACTTCGTCAGCTCTGTCTCTTATACAC40.1558846453624318No Hit
ACTTCGTCAGCTCCTGTCTCTTATACA30.11691348402182386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGC2500.021.05
TTTTTTT1450.021.021
GTTTTTT652.1827873E-1121.020
ACTTCGT2100.021.01
TCAGCTG701.8189894E-1221.07
TCAGCTA402.719351E-621.07
TCGTCAG2500.021.04
CTTCGTC2100.021.02
ATTTTTT602.3101165E-1021.020
GTCAGCT2550.021.06
CAGCTCG250.002551460920.9999988
TTCGTCA2200.020.9999983
TCAGCTT950.020.9999987
TCAGCTC502.602792E-820.9999987
GATTTTT250.002551460920.99999819
CAGCTTG452.6855378E-720.9999988
CGTTTTT250.002551460920.99999819
CAGCTGG250.002551460920.9999988
CAGCTAG300.007349053417.58
CTGTCTC650.003087741811.30769221
Produced by FastQC (version 0.11.2)