FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_38.3510000005ff40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_38.3510000005ff40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA900.6421232876712328No Hit
TGTTGCCGAACAGCTGTCTCTTATACA220.1569634703196347No Hit
TGTTGCCGAACACCTGTCTCTTATACA190.1355593607305936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT557.006747E-921.05734818
GGGTTTT800.021.05734818
GCCTTTT250.003166953621.05734818
GCGATTT250.003166953621.05734817
AATTTTT404.6953537E-621.05734819
TGATTTT303.6047434E-421.05734818
CGCTTTT250.003166953621.05734818
GCGGTTT303.6047434E-421.05734817
TGTTTTT1400.021.05734819
GTATTTT303.6047434E-421.05734818
GGATTTT303.6047434E-421.05734818
CATTTTT800.021.05734819
CCGTTTT250.003166953621.05734818
TAGTTTT303.6047434E-421.05734818
GAGTTTT354.1113497E-521.05734818
ACTTTTT506.132541E-821.05734819
CGGGTTT250.003166953621.05734817
GTCTTTT303.6047434E-421.05734818
CCTTTTT659.094947E-1121.05734819
TATTTTT751.8189894E-1221.05734819
Produced by FastQC (version 0.11.2)