FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_37.3510000005ff26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_37.3510000005ff26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA190.7404520654715511No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC50.19485580670303976No Hit
CTCTAGTACCGACCTGTCTCTTATACA50.19485580670303976No Hit
CTCTAGTACCGAGCTGTCTCTTATACA30.11691348402182386No Hit
CTCTAGTACCGAACCTGTCTCTTATAC30.11691348402182386No Hit
CTCTAGTACCGACGCTGTCTCTTATAC30.11691348402182386No Hit
CTCTAGTACCGATCTGTCTCTTATACA30.11691348402182386No Hit
CTCTAGTACCGCTGTCTCTTATACCCA30.11691348402182386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA2500.021.06
TTTTTTT1600.021.021
GTTTTTT750.021.020
GGTTTTT402.719351E-621.019
ACCGAGC302.6474395E-421.08
AGTACCG2400.021.05
TAGTACC2400.021.04
TACCGAG1250.021.07
CCGAGTG302.6474395E-421.09
CTAGTAC2350.021.03
ATTTTTT402.719351E-621.020
TCTAGTA2250.020.9999982
CTCTAGT2250.020.9999981
ACCGAGT502.602792E-820.9999988
ACCGAGG250.002551460920.9999988
ACCGACG250.002551460920.9999988
TACCGAT502.602792E-820.9999987
TACCGAC502.602792E-820.9999987
TACCGAA250.002551460920.9999987
CTTTTTT452.6855378E-720.99999820
Produced by FastQC (version 0.11.2)