FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_34.3510000005feba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_34.3510000005feba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24614
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC1890.7678556918826683No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC730.29657918257902005No Hit
ACTCGTCTCGATGCTGTCTCTTATACA430.17469732672462826No Hit
ACTCGTCTCGATGGCTGTCTCTTATAC330.134070041439831No Hit
ACTCGTCTCGATCCTGTCTCTTATACA290.1178191273259121No Hit
ACTCGTCTCGCTGTCTCTTATACACAT280.11375639879743235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGG303.7848606E-421.00000210
GATGGGC303.7848606E-421.00000210
GATGGGA303.7848606E-421.00000210
GATGGAG303.7848606E-421.00000210
ATGGGCG303.7848606E-421.00000211
CAGTTTT651.07320375E-1021.00000218
GTCATTT303.7848606E-421.00000217
CGATATT405.0647177E-621.0000029
CGATAGT303.7848606E-421.0000029
CGATAGG651.07320375E-1021.0000029
CGATACG303.7848606E-421.0000029
CGATAAG405.0647177E-621.0000029
AAATTTT303.7848606E-421.00000218
TCGATCG2100.021.0000028
TCGATCA1200.021.0000028
TCGATAC609.276846E-1021.0000028
TTTCTGT303.7848606E-421.00000221
CGCTTTT405.0647177E-621.00000218
GATGCGG651.07320375E-1021.00000210
GATGTGG303.7848606E-421.00000210
Produced by FastQC (version 0.11.2)