FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_33.3510000005fe91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_33.3510000005fe91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA240.911854103343465No Hit
AACGGAATGGCTCCTGTCTCTTATACA120.4559270516717325No Hit
AACGGAATGGCTGCTGTCTCTTATACA110.4179331306990881No Hit
AACGGAATGGCTCTGTCTCTTATACAC100.3799392097264438No Hit
AACGGAATGGCTGTCTCTTATCCACAT80.303951367781155No Hit
AACGGAATGGCTTCTGTCTCTTATACA70.26595744680851063No Hit
AACGGAATGGCTCTGTCTCTTCTACAC60.22796352583586624No Hit
AACGGAATGGCTACTGTCTCTTATACA50.1899696048632219No Hit
AACGGAATGGCTGTCTCTTATACACAT50.1899696048632219No Hit
AACGGAATGGCTGCTGTCTCTTATACC40.1519756838905775No Hit
AACGGAATGGCCTGTCTCTTATACCCA30.11398176291793312No Hit
AACGGAATGGCTCAGATCTGTTTTTTT30.11398176291793312No Hit
AACGGAATGGCTGAGGGGCGTTTTTTT30.11398176291793312No Hit
AACGGAATGGCTGGGTGCCTGTCTCTT30.11398176291793312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC750.021.07
GAATGGC2600.021.05
CGGAATG2400.021.03
AATGGCT2600.021.06
AACGGAA2300.021.01
GGAATGG2600.021.04
AGTTTTT352.7348166E-521.019
ACGGAAT2300.021.02
TGGCTGG352.7348166E-521.08
TGGCTCG352.7348166E-521.08
ATGGCTT452.7341775E-720.9999987
ATGGCTG950.020.9999987
ATGGCTA452.7341775E-720.9999987
ATTTTTT552.5411282E-920.99999820
TGGCTGT250.002565891720.9999988
TGGCTGC250.002565891720.9999988
TTTTTTT1800.020.41666421
GTTTTTT1000.019.94999920
GGTTTTT507.570725E-718.919
Produced by FastQC (version 0.11.2)