FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_31.3510000005fe6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_31.3510000005fe6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21650
Sequences flagged as poor quality0
Sequence length27
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT303.711273E-421.04398221
CTCTGTC250.00323550521.04398220
CAGCCAT354.3481647E-520.9953821
GTGTTTT609.113137E-1020.99538218
AGCTTTT303.7700866E-420.99538218
AGCCATC354.3481647E-520.9953822
GCAAGTG609.113137E-1020.9953829
GCAAGCT455.8241676E-720.9953829
GCAAGCG303.7700866E-420.9953829
GCAAGAT354.3481647E-520.9953829
TGATTTT354.3481647E-520.99538218
TGACATC609.113137E-1020.9953822
GTATTTT303.7700866E-420.99538218
CAACAGT303.7700866E-420.99538210
GCAATTG455.8241676E-720.9953829
GCAATTA303.7700866E-420.9953829
GCAACGT303.7700866E-420.9953829
GCAACGA455.8241676E-720.9953829
GCAATGT354.3481647E-520.9953829
ACATCGC19850.020.9953824
Produced by FastQC (version 0.11.2)