FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_30.3510000005fe41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_30.3510000005fe41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC280.7210919392222509No Hit
AGTGTGACGAACCTGTCTCTTCTACAC210.5408189544166881No Hit
AGTGTGACGAACTGTCTCTTATACACA60.1545197012619109No Hit
AGTGTGACGAACGGGGGGCGTTTTTTT50.12876641771825909No Hit
AGTGTGACGAACGGGGCCTGTCTCTTA40.10301313417460728No Hit
AGTGTGACGAACGGGGGGGATTTTTTT40.10301313417460728No Hit
AGTGTGACGAACGCCTGTCTCTTATAC40.10301313417460728No Hit
AGTGTGACGAACGCGGGGGGTTTTTTT40.10301313417460728No Hit
AGTGTGACGAACGGCTGTCTCTTATAC40.10301313417460728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG750.021.0000028
ATTTTTT950.021.00000220
TGACGAA3800.021.0000025
GGGTTTT250.00281337121.018
TTTTTTT2750.021.021
GAACGGG250.00281337121.09
GAACGGC250.00281337121.09
GTTTTTT1200.021.020
CGAACTG353.260411E-521.08
CGAACTC353.260411E-521.08
CGAACGC303.0402135E-421.08
CGAACGA250.00281337121.08
CGAACCG604.0017767E-1021.08
CGAACAA250.00281337121.08
TATTTTT250.00281337121.019
GATTTTT353.260411E-521.019
GACGAAC3850.021.06
GTGACGA3750.021.04
GCTTTTT353.260411E-521.019
AGTTTTT250.00281337121.019
Produced by FastQC (version 0.11.2)