FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_3.3510000005fae5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_3.3510000005fae5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC672.545592705167173No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC230.8738601823708206No Hit
GAGGAACAGAGCTGTCTCTTATACACA170.6458966565349544No Hit
GAGGAACAGAGCTGTCTCTTCTACACA50.1899696048632219No Hit
GAGGAACAGAGTGGGGGCTGTCTCTTA50.1899696048632219No Hit
GAGGAACAGAGTAGCTGTCTCTTATAC40.1519756838905775No Hit
GAGGAACAGAGTACCTGTCTCTTATAC40.1519756838905775No Hit
GAGGAACAGAGTGGGCGGCTGTCTCTT40.1519756838905775No Hit
GAGGAACAGAGCTGTCTCTTATACACC40.1519756838905775No Hit
GAGGCACAGAGTCTGTCTCTTCTACAC30.11398176291793312No Hit
GAGGAACAGAGTGCCTGTCTCTTATAC30.11398176291793312No Hit
GAGGAACAGAGTAGGGCCTGTCTCTTA30.11398176291793312No Hit
GAGGAACAGAGTCGGGCCTGTCTCTTA30.11398176291793312No Hit
GAGGAACAGAGTGGGACCGATTTTTTT30.11398176291793312No Hit
GTGGAACAGAGTCTGTCTCTTATACAC30.11398176291793312No Hit
GAGGAACAGAGTGAGGGCTGTCTCTTA30.11398176291793312No Hit
GAGGAACAGAGTGTGTCTGCTTTTTTT30.11398176291793312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGT2550.021.06
TTTTTTT1400.021.021
GAGTCTG352.7348166E-521.09
CAGAGTT402.7580645E-621.07
CAGAGTC701.8189894E-1221.07
AGAGTTG302.6687197E-421.08
AGAGTGG302.6687197E-421.08
AGAGTGC302.6687197E-421.08
AGAGTCT352.7348166E-521.08
AGAGTAG352.7348166E-521.08
GGAACAG2300.021.03
GAGGAAC2200.020.9999981
GTTTTTT452.7341775E-720.99999820
CATTTTT250.002565891720.99999819
AGGAACA2200.020.9999982
CAGAGTG950.020.9999987
CAGAGTA502.6613634E-820.9999987
GAACAGA2450.020.9999984
AACAGAG2450.020.9999985
AGTCTGT250.002565891720.99999810
Produced by FastQC (version 0.11.2)