FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_27.3510000005fde2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_27.3510000005fde2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5396
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCAACGAGCTGTCTCTTATACA80.14825796886582654No Hit
TTCGACCAACGACCTGTCTCTTATACA80.14825796886582654No Hit
TTCGACCAACGACTGTCTCTTATACAC70.12972572275759822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATG555.005859E-921.0000028
GCGACCA403.9442875E-621.0000022
AACGAAG353.6017373E-521.0000028
TTTTTTT4200.021.00000221
TGTTTTT403.9442875E-621.00000219
TGCGACC403.9442875E-621.0000021
ACTTTTT403.9442875E-621.00000219
CCTTTTT353.6017373E-521.00000219
TATTTTT403.9442875E-621.00000219
ATTTTTT1050.021.00000220
CGACCAA4950.021.0000023
CCAACGA5350.021.06
TCGACCA4450.021.02
AACGATT303.274146E-421.08
AACGATA303.274146E-421.08
AACGAGG750.021.08
AACGAGC303.274146E-421.08
AACGACT454.296744E-721.08
TTCGACC4450.021.01
GTTTTTT1700.021.020
Produced by FastQC (version 0.11.2)