FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_25.3510000005fdaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_25.3510000005fdaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC150.27462467960454046No Hit
CTTGGTTCGTTGCTGTCTCTTCTACAC60.10984987184181619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTTT555.0622475E-921.00000219
CTTGGTT4150.021.0000021
CGTGGTT454.329304E-721.0000021
TCGTTGT900.021.0000027
TCGTTGC1100.021.0000027
GTGGTTC454.329304E-721.0000022
CGTTGTG454.329304E-721.0000028
CGTTGGG555.0622475E-921.0000028
CGTTGGC454.329304E-721.0000028
CTTTTTT1100.021.00000220
CGTTTTT454.329304E-721.00000219
TGGTTCG4900.021.03
GTGTTTT303.2854883E-421.018
GGGTTTT250.002972578421.018
TTCGTTG5400.021.06
TCGGTTC250.002972578421.02
GTTTTTT2300.021.020
GGTTTTT850.021.019
GGTTCGT5400.021.04
CCTTTTT303.2854883E-421.019
Produced by FastQC (version 0.11.2)