FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_24.3510000005fd85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_24.3510000005fd85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11120
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC1151.0341726618705036No Hit
GTGTAGTGTGGCTGTCTCTTATACACA1050.9442446043165468No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC660.5935251798561151No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC550.49460431654676257No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC480.4316546762589928No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC460.4136690647482014No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC400.3597122302158274No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC340.3057553956834532No Hit
GTGTAGTGTGGACCTGTCTCTTATACA320.28776978417266186No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA300.2697841726618705No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC240.2158273381294964No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC240.2158273381294964No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC230.2068345323741007No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT180.1618705035971223No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC180.1618705035971223No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC150.13489208633093525No Hit
GTGTAGTGTGGATACTGTCTCTTATAC140.12589928057553956No Hit
GTGTAGTGTGGCTGTCTCTTATACCCA140.12589928057553956No Hit
GTGTAGTGTGGATCTGTCTCTTATACA130.11690647482014388No Hit
GTGTAGTGTGGACTCTGTCTCTTATAC130.11690647482014388No Hit
GTGTAGTGTGGAAACTGTCTCTTATAC120.1079136690647482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTG10400.021.0000024
TGGAGGA556.8266672E-921.0000029
TGTGGAG4550.021.0000027
GTGGATC658.731149E-1121.0000028
GTGGAGA658.731149E-1121.0000028
GTAGTGT10200.021.0000023
TTGTTTT303.6059468E-421.018
TGGTTTT354.0984996E-521.018
GTGTTTT607.730705E-1021.018
GGGTTTT709.094947E-1221.018
GTGTCGT404.660331E-621.01
AATTTTT303.6059468E-421.019
TTTCTGT250.003176189521.021
GTCGTGT404.660331E-621.03
GGGTAGT506.017581E-821.01
TTTTTTT6950.021.021
TGTTTTT1200.021.019
GGATTTT250.003176189521.018
GGTTTTT1400.021.019
CATTTTT455.2978794E-721.019
Produced by FastQC (version 0.11.2)