FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_23.3510000005fd6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_23.3510000005fd6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11123
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA660.5933650993437022No Hit
ATCTGCTATCCTGTCTCTTATACACAT310.27870178908567833No Hit
ATCTGCTATCCTGTCTCTTATCCACAT140.12586532410320958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTTT1850.021.08597220
TTGTTTT354.112089E-520.99099218
TGGTTTT354.112089E-520.99099218
GTGTTTT556.859409E-920.99099218
GGGTTTT751.8189894E-1220.99099218
AGCTTTT354.112089E-520.99099218
TTATTTT404.6777022E-620.99099218
GGATTTT303.6164199E-420.99099218
CATTTTT354.112089E-520.99099219
GAGTTTT556.859409E-920.99099218
ACTTTTT303.6164199E-420.99099219
GCTATCC10950.020.9909925
TCTGCTA9800.020.9909922
TCCGGTG303.6164199E-420.9909929
TCCGGGC303.6164199E-420.9909929
TCCGGAG303.6164199E-420.9909929
TATCCGT2600.020.9909927
TATCCGG3900.020.9909927
TATCCGA2100.020.9909927
GATTTTT658.731149E-1120.99099219
Produced by FastQC (version 0.11.2)