FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_22.3510000005fd42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_22.3510000005fd42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15465
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCGCTGTCTCTTATACA500.3233107015842224No Hit
GTCAGAACTACCCCTGTCTCTTATACA360.23278370514064015No Hit
GTCAGAACTACCTCTGTCTCTTATACA200.12932428063368898No Hit
GTCAGAACTACCACTGTCTCTTATACA180.11639185257032007No Hit
GTCAGAACTACCGGGGGGGGTTTTTTT180.11639185257032007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT354.237154E-521.00000218
GTGTTTT303.69692E-421.00000218
GGGTTTT850.021.00000218
ACAGAAC303.69692E-421.0000022
GACAGAA303.69692E-421.0000021
TTCTGTC303.69692E-421.00000221
CCAGAAC354.237154E-521.0000022
ACCCGGG354.237154E-521.00000210
AGAACTA14650.021.0000024
GAGTTTT354.237154E-521.00000218
GCCAGAA354.237154E-521.0000021
TACCGGG1400.021.0000029
TACCGAG850.021.0000029
TACCGAA354.237154E-521.0000029
CCTTTTT303.69692E-421.00000219
TATTTTT303.69692E-421.00000219
TCGTTTT303.69692E-421.00000218
ATGTTTT354.237154E-521.00000218
AGGTTTT303.69692E-421.00000218
TACCCGG608.4946805E-1021.0000029
Produced by FastQC (version 0.11.2)