FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_20.3510000005fcf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_20.3510000005fcf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT741.091767483033343No Hit
AGTCTCCTACGTCTGTCTCTTATACAC650.9589849513130718No Hit
AGTCTCCTACCTGTCTCTTATCCACAT350.5163765122455002No Hit
AGTCTCCTACGTCTGTCTCTTCTACAC290.42785482443198586No Hit
AGTCTCCTACGCTGTCTCTTATACACA190.28031867807612865No Hit
AGTCTCCTACGTGGCTGTCTCTTATAC140.20655060489820007No Hit
AGTCTCCTACGTGCTGTCTCTTATACA70.10327530244910003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG250.00304848921.0000029
ACGTTTG250.00304848921.0000029
TTTTTTT4650.021.00000221
GTTTTTT2000.021.00000220
TACGTTT555.677066E-921.0000028
TACGTTG555.677066E-921.0000028
TACGTAG250.00304848921.0000028
CTACGTT1500.021.0000027
GCTTTTT555.677066E-921.00000219
ATTTTTT1500.021.00000220
AGTTTTT250.00304848921.00000219
ACGTGGC250.00304848921.0000029
AGCCTCC404.2193715E-621.01
GGGTTTT303.404093E-421.018
GCCTCCT404.2193715E-621.02
CCTACGT6550.021.06
AATTTTT303.404093E-421.019
ACGTCGT404.2193715E-621.09
TCCTACG6350.021.05
CTCCTAC6500.021.04
Produced by FastQC (version 0.11.2)