FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_2.3510000005facb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_2.3510000005facb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAAGGCTGTCTCTTATA30.24No Hit
GGTAGTGAGCTAGGAGGGAATTTTTTT30.24No Hit
GGTAGTGAGCTAGAGGGCTGTCTCTTA30.24No Hit
GGTAGTGAGCTATGTGAATGTTTTTTT20.16No Hit
GGTAGTGAGCTAGGTCAGCGTTTTTTT20.16No Hit
GGTAGTGAGCTAATGGGAGATTTTTTT20.16No Hit
GGTAGTGAGCTAGTGGCTGTCTCTTAT20.16No Hit
GGTAGTGAGCTATGCACTTATTTTTTT20.16No Hit
GGTAGTGAGCTATCGGGGTCTGTCTCT20.16No Hit
GGTAGTGAGCTAGGGGGCTGTCTGTCT20.16No Hit
GGTAGTGAGCTAGGGGCGGCTTTTTTT20.16No Hit
GGTAGTGAGCTAGGAGGGCTGTCTCTT20.16No Hit
GGTAGTGAGCTAGGCGATTGTTTTTTT20.16No Hit
GGTAGTGAGCTAGCCTTGCATTTTTTT20.16No Hit
GGTAGTGAGCTAGTGAGAGATTTTTTT20.16No Hit
GGTAGTGAGCTAGGGCGGCGTTTTTTT20.16No Hit
GGTAGTGAGCTATGCCTGTCTCTTATA20.16No Hit
GGTAGTGAGCTATAGCTGTCTCTTATA20.16No Hit
GGTAGTGAGCTACGTATTAATTTTTTT20.16No Hit
GGTAGTGAGCTAGGGGAGGGTTTTTTT20.16No Hit
GGTAGTGAGCTATCGGTGTGTTTTTTT20.16No Hit
GGTAGTGAGCTAGGGGCTGTCTCTTAT20.16No Hit
GGTAGTGAGCTATAGGCTGTCTCTTAT20.16No Hit
GGTAGTGAGCTAAGCCCTGTCTCTTAT20.16No Hit
GGTAGTGAGCTAGGGCTGTCTCTTATA20.16No Hit
GGTAGTGAGCTAGTCTAAGGTTTTTTT20.16No Hit
GGTAGTGAGCTAAAGGCTGTCTCTTAT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGCT1200.021.0000025
AGTGAGC1200.021.0000024
TGAGCTA1200.021.0000026
TTTTTTT750.021.021
GTTTTTT351.44566875E-521.020
GAGCTAG351.44566875E-521.07
GAGCTAC401.1890661E-621.07
GGTAGTG1050.021.01
ATTTTTT351.44566875E-521.020
TAGTGAG1000.020.9999983
GAGCTAT250.001853412620.9999987
GTAGTGA1000.020.9999982
Produced by FastQC (version 0.11.2)