FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_19.3510000005fce3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_19.3510000005fce3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9739
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC180.18482390389156997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA9350.021.0000024
AATTTTT404.5584566E-621.00000219
TGTTTTT800.021.00000219
CATTCGG404.5584566E-621.0000029
ACTTTTT404.5584566E-621.00000219
GATTTTT658.185452E-1121.00000219
GCATTTG404.5584566E-621.0000028
GCTTTTT404.5584566E-621.00000219
GCATTCT404.5584566E-621.0000028
GCATTCG800.021.0000028
GCATTCA404.5584566E-621.0000028
GCATTAC404.5584566E-621.0000028
GTGTTTT303.5599733E-421.018
GGGTTTT354.028823E-521.018
CATTGTG303.5599733E-421.09
CATTGTC250.00314726521.09
CATTGGT354.028823E-521.09
CATTGGC354.028823E-521.09
CATTGGA250.00314726521.09
CATTGCT303.5599733E-421.09
Produced by FastQC (version 0.11.2)