FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_18.3510000005fcc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_18.3510000005fcc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18492
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA1480.800346095608912No Hit
GCATTGGATACCGGCTGTCTCTTATAC910.49210469392169587No Hit
GCATTGGATACCCTGTCTCTTATACAC770.4163962794722042No Hit
GCATTGGATACCAGCTGTCTCTTATAC600.32446463335496434No Hit
GCATTGGATACCGTCTGTCTCTTATAC500.2703871944624703No Hit
GCATTGGATACCTGCTGTCTCTTATAC480.25957170668397145No Hit
GCATTGGATACCCGCTGTCTCTTATAC450.24334847501622323No Hit
GCATTGGATACCATCTGTCTCTTATAC400.2163097555699762No Hit
GCATTGGATACCGCCTGTCTCTTATAC380.2054942677914774No Hit
GCATTGGATACCACCTGTCTCTTATAC360.1946787800129786No Hit
GCATTGGATACCGACTGTCTCTTATAC340.18386329223447978No Hit
GCATTGGATACCCCCTGTCTCTTATAC310.16764006056673156No Hit
GCATTGGATACTGTCTCTTATACACAT270.14600908500973395No Hit
GCATTGGATACCCCTGTCTCTTATACA260.14060134112048453No Hit
GCATTGGATACCTACTGTCTCTTATAC250.13519359723123514No Hit
GCATTGGATACCCTGTCTCTTCTACAC250.13519359723123514No Hit
GCATTGGATACCTCCTGTCTCTTATAC230.12437810945273632No Hit
GCATTGGATACCTTCTGTCTCTTATAC210.11356262167423752No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT608.8220986E-1021.00000218
ACCGGGA303.7351716E-421.00000210
ACCGGCG303.7351716E-421.00000210
GGGTTTT751.8189894E-1221.00000218
ACCGGAC303.7351716E-421.00000210
AATTTTT850.021.00000219
TGTTTTT1500.021.00000219
ACTTTTT608.8220986E-1021.00000219
ACCGAGG303.7351716E-421.00000210
ACCTGGT303.7351716E-421.00000210
TACCGTG608.8220986E-1021.0000029
TACCGTA608.8220986E-1021.0000029
TACCGCC303.7351716E-421.0000029
ACCCTGT303.7351716E-421.00000210
TCGTTTT303.7351716E-421.00000218
GCGTTTT303.7351716E-421.00000218
TTTGGAT751.8189894E-1221.0000023
ATACCTG1700.021.0000028
ATACCTC850.021.0000028
TACCCGT303.7351716E-421.0000029
Produced by FastQC (version 0.11.2)