FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_11.3510000005fbd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_11.3510000005fbd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGTAGCTGTCTCTTATAC90.1647748077627243No Hit
AAGTGCCTCAGCTGTCTCTTATACACA90.1647748077627243No Hit
AAGTGCCTCAGTCCTGTCTCTTATACA60.10984987184181619No Hit
AAGTGCCTCAGTCTGTCTCTTCTACAC60.10984987184181619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTTG454.329304E-721.0000028
TCAGTAT454.329304E-721.0000028
TGGTTTT303.2854883E-421.018
CAGTCTC250.002972578421.09
CAGTTTG250.002972578421.09
GCCTCAG5300.021.05
CAGTTCG250.002972578421.09
AATTTTT353.6184807E-521.019
TGTTTTT504.6964487E-821.019
GGTTTTT605.4205884E-1021.019
CATTTTT303.2854883E-421.019
ACTTTTT250.002972578421.019
TCAGTTT504.6964487E-821.08
TCAGTGT705.456968E-1221.08
TCAGTGG605.4205884E-1021.08
TCAGTGA303.2854883E-421.08
TCAGTCT403.9680217E-621.08
TCAGTCG353.6184807E-521.08
TCAGTCC303.2854883E-421.08
TGCCTCA5300.021.04
Produced by FastQC (version 0.11.2)