FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_10.3510000005fbbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_10.3510000005fbbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC471.7857142857142856No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC281.0638297872340425No Hit
ACGTCTCTGCCTGTCTCTTATACACAT150.5699088145896657No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT80.303951367781155No Hit
ACGTCTCTGCATACTTCGTGTTTTTTT30.11398176291793312No Hit
ACGTCTCTGCATACGCGAGATTTTTTT30.11398176291793312No Hit
ACGTCTCTGCATAGTGGCTGTCTCTTA30.11398176291793312No Hit
ACGTCTCTGCATCGCAGGGGTTTTTTT30.11398176291793312No Hit
ACGTCTCTGCATGGGGGTGGTTTTTTT30.11398176291793312No Hit
ACGTCTCTGCATCTGTCTCTTCTACCC30.11398176291793312No Hit
ACGTATCTGCATCTGTCTCTTCTACAC30.11398176291793312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGCA2550.021.05
TGCATGT302.6687197E-421.08
TGCATGG602.382876E-1021.08
TGCATGA302.6687197E-421.08
ACGTCTC2500.021.01
GGTTTTT302.6687197E-421.019
TCTGCAT2600.021.06
GTCTCTG2550.021.03
TCTCTGC2550.021.04
CTGCATG1300.021.07
CTGCATA302.6687197E-421.07
ATTTTTT701.8189894E-1221.020
CGTCTCT2500.021.02
TGCATCC250.002565891720.9999988
TATTTTT250.002565891720.99999819
CTGCATT452.7341775E-720.9999987
CTGCATC552.5411282E-920.9999987
GCTTTTT250.002565891720.99999819
CTTTTTT552.5411282E-920.99999820
TTTTTTT2100.020.521
Produced by FastQC (version 0.11.2)