FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate7_1.3510000005faa2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate7_1.3510000005faa2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT220.5572441742654508No Hit
GTGTCTGCTCCTGTCTCTTATCCACAT70.1773049645390071No Hit
GTGTCTGCTCATAGGCTGTCTCTTATA40.10131712259371835No Hit
GTGTCTGCTCATGGGGGGGATTTTTTT40.10131712259371835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTG3400.021.01
GGGTTTT303.050626E-421.018
TGTCTGC3400.021.02
CCTGTCT250.002820193521.020
TTTTTTT2500.021.021
TGTTTTT353.275441E-521.019
GTTTTTT1200.021.020
GGTTTTT403.4876957E-621.019
GTCTGCT3650.021.03
TCTGCTC3750.021.04
TGCTCAT3800.021.06
TCATGAC303.050626E-421.09
GATTTTT303.050626E-421.019
CTGCTCA3750.021.05
CTCATTG403.4876957E-621.08
TCATCGG250.002820193521.09
CTCATGT403.4876957E-621.08
CTCATGG353.275441E-521.08
CTCATGA604.0563464E-1021.08
CTCATCG303.050626E-421.08
Produced by FastQC (version 0.11.2)