FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_94.3510000005fa45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_94.3510000005fa45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89758
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA2540.2829831324227367No Hit
TACTGCAAGGAGGCTGTCTCTTATACA1250.13926335257024444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTTT4100.021.0089219
GGATCTG651.2187229E-1021.0089218
GAATTTT651.2187229E-1021.0089218
TGGTTTT1150.021.00891918
TCCTTTT405.299791E-621.00891918
GTGTTTT1600.021.00891918
GCCTTTT900.021.00891918
CAGTTTT1050.021.00891918
GGGTTCT507.335257E-821.00891918
AAATTTT405.299791E-621.00891918
TGCGTTT456.225855E-721.00891917
CCAGTTT250.003347626421.00891917
AATTCTG303.8836268E-421.00891919
AATTTTT2200.021.00891919
ACGTTTT303.8836268E-421.00891918
GGCGTTT800.021.00891917
TATGTTT303.8836268E-421.00891917
TTATCTG250.003347626421.00891918
TGATTTT900.021.00891918
CTCTTTT250.003347626421.00891918
Produced by FastQC (version 0.11.2)