FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_91.3510000005f9d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_91.3510000005f9d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55277
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA4420.7996092407330355No Hit
CTAGCTGGATGTCTGTCTCTTATACAC1340.24241547117245874No Hit
CTAGCTGGATCTGTCTCTTATACACAT690.12482587694701232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTG405.258413E-621.010
TGTTGTC303.8686645E-421.010
AGGGGTT303.8686645E-421.016
TTGTTTT850.021.018
TGGTTTT1800.021.018
GGATGTG22550.021.07
GGATGTC11250.021.07
GGATGTA9700.021.07
TGTGAGC405.258413E-621.010
TGTGACT250.003339771421.010
TGTTGGC601.0059011E-921.010
TGTTGGA456.1620085E-721.010
TGTTGCC456.1620085E-721.010
TGTTGAG250.003339771421.010
TGTTGAA250.003339771421.010
GGGTTTT2600.021.018
GCCTTTT601.0059011E-921.018
CAGTTTT900.021.018
GTCATTT250.003339771421.017
GGCATTT354.501662E-521.017
Produced by FastQC (version 0.11.2)