FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_9.3510000005ef93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_9.3510000005ef93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25665
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTACGTAGGCTGTCTCTTATACA2020.7870640950711084No Hit
TACGGTACGTAGCCTGTCTCTTATACA1000.3896356906292617No Hit
TACGGTACGTAGCTGTCTCTTATACAC950.3701539060977985No Hit
TACGGTACGTAGTCTGTCTCTTATACA760.29612312487823883No Hit
TACGGTACGTAGACTGTCTCTTATACA590.22988505747126436No Hit
TACGGTACGTAGGGCTGTCTCTTATAC380.14806156243911942No Hit
TACGGTACGTAGGGGCTGTCTCTTATA320.12468342100136372No Hit
TACGGTACGTAGCTGTCTCTTCTACAC320.12468342100136372No Hit
TACGGTACGTAGAGCTGTCTCTTATAC310.12078706409507112No Hit
TACGGTACGTAGGCTGTCTCTTATCCC270.10520163646990065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA23000.021.0390621
TACGCTA250.0032562521.0390621
TACGATA404.9970513E-621.0390621
TTCGGTA900.021.0390621
TATGGTA751.8189894E-1221.0390621
ACGTAGG10700.020.9980537
CGTAGTC850.020.9980538
CGTAGAA1300.020.9980538
GTAGGGT651.09139364E-1020.9980539
AAGTTTT651.09139364E-1020.99805318
TTGTTTT354.384743E-520.9980518
GTAGAGC558.007191E-920.998059
GTAGAGA354.384743E-520.998059
GTGTTTT558.007191E-920.9980518
CAGTTTT455.9052945E-720.9980518
TAGGAGG455.9052945E-720.9980510
AATTTTT1500.020.9980519
TGGTACG751.8189894E-1220.998053
TGTCTGT354.384743E-520.9980519
TTATTTT354.384743E-520.9980518
Produced by FastQC (version 0.11.2)