FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_89.3510000005f996.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_89.3510000005f996.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61859
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC3000.48497389223880116No Hit
TCAACCGATAGGGCTGTCTCTTATACA680.10992741557412826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGA55350.021.0145652
TCAACCG55350.021.0145651
AACCGAT56650.021.0145633
CACCGAT1200.021.0145633
ACCCGAT900.021.0145633
TCTACCG1900.021.0145631
CCACCGA1200.021.0145632
TCAAACG850.021.0145631
TAACCGA1250.021.0145632
TACCGAT1900.021.0145633
TCCACCG1200.021.0145631
TCACCCG900.021.0145631
TTAACCG1250.021.0145631
AAACGAT850.021.0145633
CAAACGA850.021.0145632
CTACCGA1900.021.0145632
TGGTTTT751.8189894E-1220.99757618
TCCTTTT405.2805553E-620.99757618
TAGGATG1600.020.9975769
TAGGATA950.020.9975769
Produced by FastQC (version 0.11.2)