FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_88.3510000005f97c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_88.3510000005f97c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126484
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC5500.4348376079187881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT354.5505738E-521.0063317
TTCATTT405.3339354E-621.0063317
CCAATTT250.003357525421.0063317
TATATTT354.5505738E-521.0063317
TCGATTT405.3339354E-621.0063317
GAAATTT354.5505738E-521.0063317
TTCGTTT405.3339354E-621.0063317
GATATTT405.3339354E-621.0063317
CCAGTTT354.5505738E-521.0063317
GCGATTT651.2369128E-1021.0063317
GAAGTTT800.021.0063317
CCGCTTT354.5505738E-521.0063317
GCGGTTT1300.021.0063317
ATGGTTT850.021.0063317
CTGATTT651.2369128E-1021.0063317
GCTATTT701.4551915E-1121.0063317
ATTATTT354.5505738E-521.0063317
AACTTTT800.021.0063318
TTCCTTT250.003357525421.0063317
TGCCTTT354.5505738E-521.0063317
Produced by FastQC (version 0.11.2)