FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_86.3510000005f939.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_86.3510000005f939.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28691
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA720.2509497751908264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT1500.021.00000218
AACATGT751.8189894E-1221.0000029
GGGCTTT751.8189894E-1221.00000217
ACAGCGC303.8060837E-421.010
ACAGCGA303.8060837E-421.010
TGATGCC506.937262E-821.01
TGGTTTT800.021.018
ACAGCAG303.8060837E-421.010
TCCTTTT303.8060837E-421.018
ACAAAGG303.8060837E-421.010
AACAATG609.458745E-1021.09
AACAATA405.113514E-621.09
ACACGGG354.404872E-521.010
ACACGGA250.003301062221.010
GGGTTTT1600.021.018
GCCTTTT506.937262E-821.018
AACAAGT354.404872E-521.09
AACAAGG651.10958354E-1021.09
AACAAGC303.8060837E-421.09
AACAAGA250.003301062221.09
Produced by FastQC (version 0.11.2)