FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_84.3510000005f8f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_84.3510000005f8f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79560
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA3410.4286073403720463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT900.021.00880618
CCGTGTT250.00334442121.00880616
GTCATTT405.2875075E-621.00880617
ATCTTTT507.309245E-821.00880618
AGCTTTT1000.021.00880618
TTACTTT250.00334442121.00880617
TATACAC651.200533E-1021.00880620
CTCCTTT250.00334442121.00880617
CGCCTTT250.00334442121.00880617
TGTCTTT456.2077925E-721.00880617
AGGATTT250.00334442121.00880617
GGCGCCT250.00334442121.00880616
CCGCTTT250.00334442121.00880617
TGATTTT1300.021.00880618
GATGTTT405.2875075E-621.00880617
CGCTTTT1500.021.00880618
ATGGTTT405.2875075E-621.00880617
AGGGTTT651.200533E-1021.00880617
GCGAATT250.00334442121.00880616
GCGGTGT250.00334442121.00880615
Produced by FastQC (version 0.11.2)