FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_83.3510000005f8da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_83.3510000005f8da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32574
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA2700.8288819303739179No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA330.10130779149014552No Hit
CACAGAAGGACTGGGGCTGTCTCTTAT330.10130779149014552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGG250.003310664821.00000210
CAGTTTT456.002556E-721.00000218
ATCTTTT507.0114766E-821.00000218
ACTCGCG507.0114766E-821.00000210
AAATTTT250.003310664821.00000218
ACGTTTT507.0114766E-821.00000218
ACTGAGC250.003310664821.00000210
GGACTTC1000.021.0000028
CTGGGGG507.0114766E-821.00000211
TGATTTT456.002556E-721.00000218
GCGGTTT250.003310664821.00000217
CCGTTTT250.003310664821.00000218
ACTTTTG250.003310664821.00000210
ACTTTGG250.003310664821.00000210
ACTCATG250.003310664821.00000210
TGGGGGT250.003310664821.00000212
TAATTTT250.003310664821.00000218
GATTCTG250.003310664821.00000219
GGGGTTT507.0114766E-821.00000217
CAGGTTT250.003310664821.00000217
Produced by FastQC (version 0.11.2)