FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_82.3510000005f8b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_82.3510000005f8b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29020
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC1640.5651274982770503No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC680.2343211578221916No Hit
ACTCGTCTCGATGCTGTCTCTTATACA340.1171605789110958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGC558.109055E-921.00000210
TGGTTTT850.021.00000218
CGATATT405.1171046E-621.0000029
CGATAGG850.021.0000029
TCGATGC1600.021.0000028
TCGATCC1600.021.0000028
CGCTTTT558.109055E-921.00000218
CAATTTT405.1171046E-621.00000218
GATCGGG405.1171046E-621.00000210
TAGTTTT558.109055E-921.00000218
GAGTTTT850.021.00000218
TGCTTTT558.109055E-921.00000218
GGCTTTT850.021.00000218
GTGGTTT558.109055E-921.00000217
CGATGTA558.109055E-921.0000029
CGATGGC800.021.0000029
CTGTTTT558.109055E-921.00000218
CGATGAG800.021.0000029
AGATTTT800.021.00000218
GACTTTT405.1171046E-621.00000218
Produced by FastQC (version 0.11.2)