FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_81.3510000005f897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_81.3510000005f897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61004
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA1000.1639236771359255No Hit
AACGGAATGGCTGCTGTCTCTTATACA870.1426135991082552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATTT303.8750595E-421.00000217
GGTTGTT303.8750595E-421.00000216
GCCTTTT303.8750595E-421.00000218
CCAGTTT303.8750595E-421.00000217
CCTGTTT303.8750595E-421.00000217
AACGAAA950.021.0000021
CTGGGAG303.8750595E-421.00000211
ATGGTTT303.8750595E-421.00000217
GTTTTCT303.8750595E-421.00000220
GGCTGCA1200.021.0000029
GACATTT303.8750595E-421.00000217
GCTACGG303.8750595E-421.00000210
GCTACCG303.8750595E-421.00000210
CTGGTGG303.8750595E-421.00000211
CTGGTGC303.8750595E-421.00000211
CGGAATG57750.021.0000023
GCTGCTC303.8750595E-421.00000210
GACGTTT601.0113581E-921.00000217
GCTGTGA303.8750595E-421.00000210
GCTGCCG303.8750595E-421.00000210
Produced by FastQC (version 0.11.2)