FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_80.3510000005f87d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_80.3510000005f87d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53896
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC1890.3506753747959032No Hit
GGATAGTTAGCCTGTCTCTTATACACA930.172554549502746No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC840.15585572213151255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGC558.51287E-921.0000029
AGCCCGA751.8189894E-1221.0000029
AGCCTGC751.8189894E-1221.0000029
AGCCCCT507.2312105E-821.0000029
AGCCTCG1100.021.0000029
AGCCCAC558.51287E-921.0000029
AGCCTAC250.003338682221.0000029
GAGCGTT250.003338682221.00000216
GGCATTT250.003338682221.00000217
AGCTTTT751.8189894E-1221.00000218
GCCTCGT250.003338682221.00000210
CCGTGGG507.2312105E-821.00000211
GCCTCGG507.2312105E-821.00000210
CCGTGCG250.003338682221.00000211
GCCCAGT250.003338682221.00000210
GCCCAGG250.003338682221.00000210
GCTAGTT2200.021.0000022
TTACTTT250.003338682221.00000217
TGACTTT250.003338682221.00000217
AGCCACG1000.021.0000029
Produced by FastQC (version 0.11.2)