FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_8.3510000005ef79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_8.3510000005ef79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56133
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC6591.1739974702937666No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC1750.3117595710188303No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC820.14608162756310905No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC700.12470382840753212No Hit
ACAGTGCTCATGGCTGTCTCTTATACA580.10332602925195518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA354.503253E-521.00000210
ACAGCGC354.503253E-521.0000021
ACAGTGC49350.021.0000021
GCGGGGT303.8696907E-421.00000215
ATGGGCA354.503253E-521.00000210
CATGATC354.503253E-521.0000029
ATGGGAA303.8696907E-421.00000210
CAGTTTT1200.021.00000218
GGCATTT303.8696907E-421.00000217
CATGAAG800.021.0000029
CATGAAC405.260801E-621.0000029
ACGTTTT601.0059011E-921.00000218
AGGATTT303.8696907E-421.00000217
CGGGGTT354.503253E-521.00000216
GGCGTTT601.0059011E-921.00000217
TTTCTGT800.021.00000221
ATGATGG303.8696907E-421.00000210
TATGTTT303.8696907E-421.00000217
CTCTTTT405.260801E-621.00000218
CGCTTTT701.4551915E-1121.00000218
Produced by FastQC (version 0.11.2)