FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_77.3510000005f810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_77.3510000005f810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28562
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCACTGTCTCTTATACAC2550.8927946222253343No Hit
AAGGTCCAAGCCTGTCTCTTATACACA2400.840277291506197No Hit
AAGGTCCAAGCACTGTCTCTTCTACAC1160.4061340242279952No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG390.13654505986975704No Hit
AAGGTCCAAGCACCTGTCTCTTATACA300.10503466143827463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCAA27200.021.031583
AGGTCCA26400.021.031582
TGGTCCA800.021.0315782
AGGACCA354.3531032E-521.0315782
TGTCCAA506.824666E-821.0315783
GGACCAA354.3531032E-521.0315783
ATGGTCC800.021.0315781
AAGGTCC26450.021.0315781
ATGTCCA506.824666E-821.0315782
AAGGACC354.3531032E-521.0315781
AATGTCC506.824666E-821.0315781
TGTTTTT1050.020.99474719
AGCAGTG1150.020.9947479
GTCCAAG27800.020.9947474
AGCACTA751.8189894E-1220.9947479
TCCAAGC27900.020.9947475
GCTTTTT1500.020.99474719
AAGCATT1450.020.9947478
AAGCATG1500.020.9947478
AAGCAGC1650.020.9947478
Produced by FastQC (version 0.11.2)